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Glarea Lozoyensis Atcc 20868, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit calcr polyclonal antibody  (Proteintech)
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Real-time PCR analysis of OC marker transcripts in cultures of RAW 264.7 cells treated with RANKL in combination with either RIG (RL/RIG) or RIG_LDH-GA (RL/RIG_LDH-GA) CM. For each sample treated with different volumes of RIG_LDH-GA CM, GA content is reported as the final concentration reached in the 48-well plate cell culture (240 μL/well). ( A ) Acp5, Ctsk, Mmp9 , and <t>Calcr</t> transcripts were normalized to the expression of Gapdh and reported as fold change of RIG- or RIG_LDH-GA-treated samples with respect to the normalized RANKL-differentiated calibrator (dotted line, fold change = 1). ****, p < 0.0001; RANKL/RIG- vs. RANKL/RIG_LDH-GA-treated (two-way ANOVA test). Data are represented as the mean ± SD of two replicates. ( B ) In the same settings of ( A ), the gene expression data are represented as a heat map. For each indicated volume of RIG or RIG_LDH-GA CM, the colour scale indicates the level of fold change of RANKL/RIG- or RANKL/RIG_LDH-GA-treated samples with respect to the positive control treated with RANKL alone (blue < 1, red ≥ 1).
Rabbit Calcr Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paav hsyn1 ne2m addgene  (Addgene inc)
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Real-time PCR analysis of OC marker transcripts in cultures of RAW 264.7 cells treated with RANKL in combination with either RIG (RL/RIG) or RIG_LDH-GA (RL/RIG_LDH-GA) CM. For each sample treated with different volumes of RIG_LDH-GA CM, GA content is reported as the final concentration reached in the 48-well plate cell culture (240 μL/well). ( A ) Acp5, Ctsk, Mmp9 , and <t>Calcr</t> transcripts were normalized to the expression of Gapdh and reported as fold change of RIG- or RIG_LDH-GA-treated samples with respect to the normalized RANKL-differentiated calibrator (dotted line, fold change = 1). ****, p < 0.0001; RANKL/RIG- vs. RANKL/RIG_LDH-GA-treated (two-way ANOVA test). Data are represented as the mean ± SD of two replicates. ( B ) In the same settings of ( A ), the gene expression data are represented as a heat map. For each indicated volume of RIG or RIG_LDH-GA CM, the colour scale indicates the level of fold change of RANKL/RIG- or RANKL/RIG_LDH-GA-treated samples with respect to the positive control treated with RANKL alone (blue < 1, red ≥ 1).
Paav Hsyn1 Ne2m Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paav hsyn1 ne2m  (Addgene inc)
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Real-time PCR analysis of OC marker transcripts in cultures of RAW 264.7 cells treated with RANKL in combination with either RIG (RL/RIG) or RIG_LDH-GA (RL/RIG_LDH-GA) CM. For each sample treated with different volumes of RIG_LDH-GA CM, GA content is reported as the final concentration reached in the 48-well plate cell culture (240 μL/well). ( A ) Acp5, Ctsk, Mmp9 , and <t>Calcr</t> transcripts were normalized to the expression of Gapdh and reported as fold change of RIG- or RIG_LDH-GA-treated samples with respect to the normalized RANKL-differentiated calibrator (dotted line, fold change = 1). ****, p < 0.0001; RANKL/RIG- vs. RANKL/RIG_LDH-GA-treated (two-way ANOVA test). Data are represented as the mean ± SD of two replicates. ( B ) In the same settings of ( A ), the gene expression data are represented as a heat map. For each indicated volume of RIG or RIG_LDH-GA CM, the colour scale indicates the level of fold change of RANKL/RIG- or RANKL/RIG_LDH-GA-treated samples with respect to the positive control treated with RANKL alone (blue < 1, red ≥ 1).
Paav Hsyn1 Ne2m, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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addgene addgene id 208654 208685  (Addgene inc)
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Real-time PCR analysis of OC marker transcripts in cultures of RAW 264.7 cells treated with RANKL in combination with either RIG (RL/RIG) or RIG_LDH-GA (RL/RIG_LDH-GA) CM. For each sample treated with different volumes of RIG_LDH-GA CM, GA content is reported as the final concentration reached in the 48-well plate cell culture (240 μL/well). ( A ) Acp5, Ctsk, Mmp9 , and <t>Calcr</t> transcripts were normalized to the expression of Gapdh and reported as fold change of RIG- or RIG_LDH-GA-treated samples with respect to the normalized RANKL-differentiated calibrator (dotted line, fold change = 1). ****, p < 0.0001; RANKL/RIG- vs. RANKL/RIG_LDH-GA-treated (two-way ANOVA test). Data are represented as the mean ± SD of two replicates. ( B ) In the same settings of ( A ), the gene expression data are represented as a heat map. For each indicated volume of RIG or RIG_LDH-GA CM, the colour scale indicates the level of fold change of RANKL/RIG- or RANKL/RIG_LDH-GA-treated samples with respect to the positive control treated with RANKL alone (blue < 1, red ≥ 1).
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calcr  (Proteintech)
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Real-time PCR analysis of OC marker transcripts in cultures of RAW 264.7 cells treated with RANKL in combination with either RIG (RL/RIG) or RIG_LDH-GA (RL/RIG_LDH-GA) CM. For each sample treated with different volumes of RIG_LDH-GA CM, GA content is reported as the final concentration reached in the 48-well plate cell culture (240 μL/well). ( A ) Acp5, Ctsk, Mmp9 , and <t>Calcr</t> transcripts were normalized to the expression of Gapdh and reported as fold change of RIG- or RIG_LDH-GA-treated samples with respect to the normalized RANKL-differentiated calibrator (dotted line, fold change = 1). ****, p < 0.0001; RANKL/RIG- vs. RANKL/RIG_LDH-GA-treated (two-way ANOVA test). Data are represented as the mean ± SD of two replicates. ( B ) In the same settings of ( A ), the gene expression data are represented as a heat map. For each indicated volume of RIG or RIG_LDH-GA CM, the colour scale indicates the level of fold change of RANKL/RIG- or RANKL/RIG_LDH-GA-treated samples with respect to the positive control treated with RANKL alone (blue < 1, red ≥ 1).
Calcr, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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154 calcr  (Proteintech)
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Real-time PCR analysis of OC marker transcripts in cultures of RAW 264.7 cells treated with RANKL in combination with either RIG (RL/RIG) or RIG_LDH-GA (RL/RIG_LDH-GA) CM. For each sample treated with different volumes of RIG_LDH-GA CM, GA content is reported as the final concentration reached in the 48-well plate cell culture (240 μL/well). ( A ) Acp5, Ctsk, Mmp9 , and <t>Calcr</t> transcripts were normalized to the expression of Gapdh and reported as fold change of RIG- or RIG_LDH-GA-treated samples with respect to the normalized RANKL-differentiated calibrator (dotted line, fold change = 1). ****, p < 0.0001; RANKL/RIG- vs. RANKL/RIG_LDH-GA-treated (two-way ANOVA test). Data are represented as the mean ± SD of two replicates. ( B ) In the same settings of ( A ), the gene expression data are represented as a heat map. For each indicated volume of RIG or RIG_LDH-GA CM, the colour scale indicates the level of fold change of RANKL/RIG- or RANKL/RIG_LDH-GA-treated samples with respect to the positive control treated with RANKL alone (blue < 1, red ≥ 1).
154 Calcr, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Real-time PCR analysis of OC marker transcripts in cultures of RAW 264.7 cells treated with RANKL in combination with either RIG (RL/RIG) or RIG_LDH-GA (RL/RIG_LDH-GA) CM. For each sample treated with different volumes of RIG_LDH-GA CM, GA content is reported as the final concentration reached in the 48-well plate cell culture (240 μL/well). ( A ) Acp5, Ctsk, Mmp9 , and Calcr transcripts were normalized to the expression of Gapdh and reported as fold change of RIG- or RIG_LDH-GA-treated samples with respect to the normalized RANKL-differentiated calibrator (dotted line, fold change = 1). ****, p < 0.0001; RANKL/RIG- vs. RANKL/RIG_LDH-GA-treated (two-way ANOVA test). Data are represented as the mean ± SD of two replicates. ( B ) In the same settings of ( A ), the gene expression data are represented as a heat map. For each indicated volume of RIG or RIG_LDH-GA CM, the colour scale indicates the level of fold change of RANKL/RIG- or RANKL/RIG_LDH-GA-treated samples with respect to the positive control treated with RANKL alone (blue < 1, red ≥ 1).

Journal: Biomaterials and Biosystems

Article Title: Gallic acid released by a layered double hydroxide-coated scaffold of hydroxyapatite and β-tricalcium phosphate inhibits the osteoclast formation In Vitro

doi: 10.1016/j.bbiosy.2025.100119

Figure Lengend Snippet: Real-time PCR analysis of OC marker transcripts in cultures of RAW 264.7 cells treated with RANKL in combination with either RIG (RL/RIG) or RIG_LDH-GA (RL/RIG_LDH-GA) CM. For each sample treated with different volumes of RIG_LDH-GA CM, GA content is reported as the final concentration reached in the 48-well plate cell culture (240 μL/well). ( A ) Acp5, Ctsk, Mmp9 , and Calcr transcripts were normalized to the expression of Gapdh and reported as fold change of RIG- or RIG_LDH-GA-treated samples with respect to the normalized RANKL-differentiated calibrator (dotted line, fold change = 1). ****, p < 0.0001; RANKL/RIG- vs. RANKL/RIG_LDH-GA-treated (two-way ANOVA test). Data are represented as the mean ± SD of two replicates. ( B ) In the same settings of ( A ), the gene expression data are represented as a heat map. For each indicated volume of RIG or RIG_LDH-GA CM, the colour scale indicates the level of fold change of RANKL/RIG- or RANKL/RIG_LDH-GA-treated samples with respect to the positive control treated with RANKL alone (blue < 1, red ≥ 1).

Article Snippet: The following primary antibodies were used for immunoblotting: mouse NFATc1 (7A6) monoclonal antibody (#MA3–024, Invitrogen, Carlsbad, CA, USA), rabbit c-Fos (9F6) monoclonal antibody (#2250, Cell Signaling Technology, Danvers, MA, United States), rabbit RANK antibody (#4845, Cell Signaling Technology, Danvers, MA, United States), and mouse β-tubulin (AA2) monoclonal antibody (#T8328, Sigma-Aldrich, St. Louis, MO, USA), rabbit Acp5 polyclonal antibody targeting TRAP protein (#PA5–106914, Invitrogen, Carlsbad, CA, USA), rabbit Ctsk polyclonal antibody (#PA5–102483, Invitrogen, Carlsbad, CA, USA), mouse Mmp9 (5G3) monoclonal antibody (#MA5–15886, Invitrogen, Carlsbad, CA, USA) rabbit Calcr polyclonal antibody (#20868–1-AP, Proteintech, Rosemont, IL, USA).

Techniques: Real-time Polymerase Chain Reaction, Marker, Concentration Assay, Cell Culture, Expressing, Gene Expression, Positive Control